Previous studies have demonstrated that the hydrolysis of the trypsin substrate N-benzoyl-DL-arginine-2-naphthylamide (BANA), by subgingival plaque obtained from a single site, correlates best with the numbers and proportions of spirochetes in plaque samples and may serve as an indicator of clinical disease.
removes sufficient bacteria to affect subsequent subgingival plaque sampling using a curette. In 25 subjects, one healthy, gingivitis and periodontitis site was
1. Oral Microbiol Immunol. 1987 Sep;2(3):142-4. Sampling of subgingival plaque: a comparison of two methods using darkfield microscopy.
The presence of specific bacteria in subgingival plaque has been used as an indicator of active periodontal disease. The technique of subgingival sampling may conjecturally influence the identification and enumeration of microorganisms reported. In this study, paper point sampling and scaler sampling are compared. Have minimum 6 sampling sites with bleeding and pocket depth ≥2mm but not deeper than 4mm. Exclusion Criteria: Have had a dental prophylaxis within 2 weeks of plaque sampling visits; Have taken antibiotics or used anti-gingivitis / anti-bacterial oral care products such as chlorhexidine or Listerine within 2 weeks of plaque sampling visits; Subgingival plaque samples within the deepest pocket of each tooth were taken and analysed by real-time polymerase chain reaction (PCR) for Actinobacillus actinomycetemcomitans (AA), Porphyromonas 1994-08-01 · A subgingival plaque sample was then obtained using a Gracey curette machined to a blade width of 0.5 mm to facilitate access to the subgingal area.
Supragingival plaque should be removed with cotton pellets or periodontal scalers to secure noncontaminated subgingival specimens. Blood and pus at the
Detection of subgingival periodontal pathogens--comparison of two sampling strategies. METHODS: In 100 patients with aggressive/chronic periodontitis, subgingival plaque was sampled from the deepest pockets per quadrant (MT4) and per sextant (MT6). Plaque samples were taken using two sterile paper points simultaneously. 2014-02-20 Collection of subgingival plaque samples with paper points is the common method for determination of periodontal pathogens.
For each subject, one pooled subgingival sample from the deepest pocket in each quadrant (PD ≥ 5 mm) and another from 4 healthy sites (PD ≤ 3 mm; no attachment loss) were obtained, using sterile paper points. Supragingival plaque was removed prior to sampling using sterile cotton pellets.
Subsequently, subgingival ultrasonic instrumentation (SUI) was performed by using EO or saline as the irrigation agent. After continuous mouth rinsing at home with EO or saline for 7 days, subgingival plaques … 2017-08-11 2010-11-01 Journal of Investigative and Clinical Dentistry (2010), 1, 126–132 ORIGINAL ARTICLE Oral Microbiology Detection rates of presumptive periodontal pathogens in subgingival plaque samples of untreated periodontitis using either four or six pooled samples Martin Wohlfeil, Orhan Tabakci, Rita Arndt, Peter Eickholz & Katrin Nickles Department of Periodontology, Center for Dental, Oral, and The collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria. However, recently, salivary analysis has been discussed as an advantageous future diagnostic method for periodontitis because it offers simple quantitative sampling and the possibility to assess various bacteria. Subgingival plaque samples were obtained from the three pockets with the deepest probing depths.
Sampling in three out of four quadrants was conducted wherever possible. In 25 subjects, one healthy, gingivitis and periodontitis site was sampled for GCF using a strip followed by subgingival plaque sampling with a curette. Bacterial assays indicated that GCF strips removed significant numbers of bacteria when placed intracrevicularly for 5 s. Microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis DSpace Repository JavaScript is disabled for your browser. BACKGROUND The collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. However, the collection of saliva is simple and contains pathogens of all intraoral surfaces.
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Methods: In 220 patients, c Subgingival plaque samples were taken from the mesio-buccal aspect of each tooth in each subject at each monitor-ing visit. Counts of 40 subgingival spe- cies wore determined in each plaque sample using the checkerboard DNA-DNA hybridization technique (Socran-sky et al. I994).
Sampling was always performed after standardized oral hygiene instructions over a period of 3 weeks. Prior to subgingival sampling, supragingival plaque—as disclosed by an indicator—was removed with a previously unused toothbrush and water performed by the children themselves. No toothpaste was used. Methods: In 50 patients with periodontitis, subgingival plaque was sampled from the deepest pocket of each quadrant by using paper points and by gaining saliva with saline mouthrinse.
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In this investigation, we determined whether the association between BANA hydrolysis and spirochetes could be obtained in pooled subgingival plaque samples
Sampling of subgingival plaque: a comparison of two methods using darkfield microscopy. Strand P, Palmer RM, Wilson RF. The presence of specific bacteria in subgingival plaque has been used as an indicator of active periodontal disease. The technique of subgingival sampling may conjecturally influence the identification and enumeration of microorganisms reported. In this study, paper point sampling and scaler sampling are compared.
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7 Feb 2017 The hypervariable region V4 of the 16S rDNA gene was targeted to explore richness and diversity of subgingival plaque samples [11].
1987 Sep;2(3):142-4. Sampling of subgingival plaque: a comparison of two methods using darkfield microscopy.